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Freezing Responses in DMSO-Based Cryopreservation of Human iPS Cells: Aggregates Versus Single Cells

Inadequate preservation methods of human induced pluripotent stem cells (hiPSCs) have impeded efficient reestablishment of cell culture after the freeze–thaw process. In this study, we examined roles of the cooling rate, seeding temperature, and difference between cell aggregates (3–50 cells) and si...

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Detalhes bibliográficos
Publicado no:Tissue Eng Part C Methods
Main Authors: Li, Rui, Yu, Guanglin, Azarin, Samira M., Hubel, Allison
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5946737/
https://ncbi.nlm.nih.gov/pubmed/29478388
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/ten.tec.2017.0531
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