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DMSO cryopreservation is the method of choice to preserve cells for droplet-based single-cell RNA sequencing

Combining single-cell RNA sequencing (scRNA-seq) with upstream cell preservation procedures such as cryopreservation or methanol fixation has recently become more common. By separating cell handling and preparation, from downstream library generation, scRNA-seq workflows are more flexible and manage...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wohnhaas, Christian T., Leparc, Germán G., Fernandez-Albert, Francesc, Kind, David, Gantner, Florian, Viollet, Coralie, Hildebrandt, Tobias, Baum, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6650608/
https://ncbi.nlm.nih.gov/pubmed/31337793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-46932-z
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