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TsrM as a Model for Purifying and Characterizing Cobalamin-Dependent Radical S-adenosylmethionine Methylases

Cobalamin-dependent radical S-adenosylmethionine (SAM) methylases play vital roles in the de novo biosynthesis of many antibiotics, cofactors, and other important natural products, yet remain an understudied subclass of radical SAM (RS) enzymes. In addition to a [4Fe-4S] cluster that is ligated by t...

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Bibliografski detalji
Izdano u:Methods Enzymol
Glavni autori: Blaszczyk, Anthony J., Wang, Roy X., Booker, Squire J.
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5941296/
https://ncbi.nlm.nih.gov/pubmed/28882204
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.07.007
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