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Study of protein folding under native conditions by rapidly switching the hydrostatic pressure inside an NMR sample cell

In general, small proteins rapidly fold on the timescale of milliseconds or less. For proteins with a substantial volume difference between the folded and unfolded states, their thermodynamic equilibrium can be altered by varying the hydrostatic pressure. Using a pressure-sensitized mutant of ubiqui...

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Библиографические подробности
Опубликовано в: :Proc Natl Acad Sci U S A
Главные авторы: Charlier, Cyril, Alderson, T. Reid, Courtney, Joseph M., Ying, Jinfa, Anfinrud, Philip, Bax, Adriaan
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 2018
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5939115/
https://ncbi.nlm.nih.gov/pubmed/29666248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1803642115
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