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Study of protein folding under native conditions by rapidly switching the hydrostatic pressure inside an NMR sample cell

In general, small proteins rapidly fold on the timescale of milliseconds or less. For proteins with a substantial volume difference between the folded and unfolded states, their thermodynamic equilibrium can be altered by varying the hydrostatic pressure. Using a pressure-sensitized mutant of ubiqui...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Charlier, Cyril, Alderson, T. Reid, Courtney, Joseph M., Ying, Jinfa, Anfinrud, Philip, Bax, Adriaan
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5939115/
https://ncbi.nlm.nih.gov/pubmed/29666248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1803642115
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