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Study of protein folding under native conditions by rapidly switching the hydrostatic pressure inside an NMR sample cell

In general, small proteins rapidly fold on the timescale of milliseconds or less. For proteins with a substantial volume difference between the folded and unfolded states, their thermodynamic equilibrium can be altered by varying the hydrostatic pressure. Using a pressure-sensitized mutant of ubiqui...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Charlier, Cyril, Alderson, T. Reid, Courtney, Joseph M., Ying, Jinfa, Anfinrud, Philip, Bax, Adriaan
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5939115/
https://ncbi.nlm.nih.gov/pubmed/29666248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1803642115
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