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Monitoring Hydrogen Exchange During Protein Folding by Fast Pressure Jump NMR Spectroscopy
A method is introduced that permits direct observation of the rates at which backbone amide hydrogens become protected from solvent exchange after rapidly dropping the hydrostatic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions. The method is demonstrated...
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| Опубликовано в: : | J Am Chem Soc |
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| Главные авторы: | , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2017
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5586491/ https://ncbi.nlm.nih.gov/pubmed/28766333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b06676 |
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