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An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation

Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH). Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-ty...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Biol Open
Päätekijät: Dobrzycki, Tomasz, Krecsmarik, Monika, Bonkhofer, Florian, Patient, Roger, Monteiro, Rui
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: The Company of Biologists Ltd 2018
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5936060/
https://ncbi.nlm.nih.gov/pubmed/29535102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.031096
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