Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation

DNA-fluorescence in situ hybridisation (DNA-FISH) allows visualisation of chromosome organisation and rearrangement. FISH probes are pools of short fluorescently labelled DNA fragments that are often produced from template plasmids that contain large genomic inserts. For effective sample penetration...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: McCoy, Patrick J., Costello, Anthony J., Corcoran, Niall M., Hovens, Christopher M., Clarkson, Michael J.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Biochemistry, Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6308247/
https://ncbi.nlm.nih.gov/pubmed/30596026
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.11.015
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