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An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation
Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH). Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-ty...
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| Publié dans: | Biol Open |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
The Company of Biologists Ltd
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5936060/ https://ncbi.nlm.nih.gov/pubmed/29535102 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.031096 |
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