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Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging

The ability to directly image and quantify drug-target engagement and drug distribution with subcellular resolution in live cells and whole organisms is a prerequisite to establishing accurate models of the kinetics and dynamics of drug action. Such methods would thus have far-reaching applications...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Vinegoni, Claudio, Feruglio, Paolo Fumene, Brand, Christian, Lee, Sungon, Nibbs, Antoinette E, Stapleton, Shawn, Shah, Sunil, Gryczynski, Ignacy, Reiner, Thomas, Mazitschek, Ralph, Weissleder, Ralph
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5928516/
https://ncbi.nlm.nih.gov/pubmed/28686582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.043
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