Practical spectrophotometric assay for the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase, a potential antibiotic target

A new enzymatic assay for the bacterial enzyme succinyl-diaminopimelate desuccinylase (DapE, E.C. 3.5.1.18) is described. This assay employs N(6)-methyl-N(2)-succinyl-L,L-diaminopimelic acid (N(6)-methyl-L,L-SDAP) as the substrate with ninhydrin used to detect cleavage of the amide bond of the modif...

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Publicado en:PLoS One
Autores principales: Heath, Tahirah K., Lutz, Marlon R., Reidl, Cory T., Guzman, Estefany R., Herbert, Claire A., Nocek, Boguslaw P., Holz, Richard C., Olsen, Kenneth W., Ballicora, Miguel A., Becker, Daniel P.
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2018
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5919655/
https://ncbi.nlm.nih.gov/pubmed/29698518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196010
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