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Practical spectrophotometric assay for the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase, a potential antibiotic target
A new enzymatic assay for the bacterial enzyme succinyl-diaminopimelate desuccinylase (DapE, E.C. 3.5.1.18) is described. This assay employs N(6)-methyl-N(2)-succinyl-L,L-diaminopimelic acid (N(6)-methyl-L,L-SDAP) as the substrate with ninhydrin used to detect cleavage of the amide bond of the modif...
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| Veröffentlicht in: | PLoS One |
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| Hauptverfasser: | , , , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5919655/ https://ncbi.nlm.nih.gov/pubmed/29698518 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196010 |
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