Semi‐quantitative Analysis of DNA Topoisomerase‐I mRNA Level Using Reverse Transcription‐Polymerase Chain Reaction in Cancer Cell Lines: Its Relation to Cytotoxicity against Camptothecin Derivative

Expression of DNA topoisomerase (Topo)‐I mRNA in various cancer cell lines was detected using the reverse transcription‐polymerase chain reaction (RT‐PCR) method. The cytoplasmic polyadenylated RNA isolated from cancer cell lines was reverse‐transcribed and the complementary DNA was amplified by PCR...

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Publicat a:Jpn J Cancer Res
Autors principals: Niwa, Kenji, Misao, Ryou, Hanabayashi, Takabiro, Morishita, Shigeo, Murase, Toshiko, Itoh, Miho, Itoh, Naoki, Mori, Hidehiro, Tamaya, Teruhiko
Format: Artigo
Idioma:Inglês
Publicat: Blackwell Publishing Ltd 1994
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5919567/
https://ncbi.nlm.nih.gov/pubmed/7523352
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.1994.tb02960.x
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