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Semi‐quantitative Analysis of DNA Topoisomerase‐I mRNA Level Using Reverse Transcription‐Polymerase Chain Reaction in Cancer Cell Lines: Its Relation to Cytotoxicity against Camptothecin Derivative

Expression of DNA topoisomerase (Topo)‐I mRNA in various cancer cell lines was detected using the reverse transcription‐polymerase chain reaction (RT‐PCR) method. The cytoplasmic polyadenylated RNA isolated from cancer cell lines was reverse‐transcribed and the complementary DNA was amplified by PCR...

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Bibliografske podrobnosti
izdano v:Jpn J Cancer Res
Main Authors: Niwa, Kenji, Misao, Ryou, Hanabayashi, Takabiro, Morishita, Shigeo, Murase, Toshiko, Itoh, Miho, Itoh, Naoki, Mori, Hidehiro, Tamaya, Teruhiko
Format: Artigo
Jezik:Inglês
Izdano: Blackwell Publishing Ltd 1994
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5919567/
https://ncbi.nlm.nih.gov/pubmed/7523352
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.1994.tb02960.x
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