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Transforming Activity of a Synthetic c‐Ha‐ras Gene Containing O(6)‐Methylguanine in Codon 12

A mutagenic DNA‐adduct, O(6)‐methylguanine, was introduced into codon 12 of the synthetic c‐Ha‐ras gene by cassette mutagenesis. Transfection of this modified ras gene into normal NIH3T3 cells by the calcium phosphate procedure resulted in significant induction of focus formation. The ras gene inser...

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Bibliografske podrobnosti
izdano v:Jpn J Cancer Res
Main Authors: Kamiya, Hiroyuki, Miura, Kazunobu, Ohtomo, Noriko, Nishimura, Susumu, Ohtsuka, Eiko
Format: Artigo
Jezik:Inglês
Izdano: Blackwell Publishing Ltd 1991
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5918606/
https://ncbi.nlm.nih.gov/pubmed/1938604
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.1991.tb01934.x
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