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Hydrogen Deuterium Exchange Mass Spectrometry of Oxygen Sensitive Proteins

The protocol detailed here describes a way to perform hydrogen deuterium exchange coupled to mass spectrometry (HDX-MS) on oxygen sensitive proteins. HDX-MS is a powerful tool for studying the protein structure-function relationship. Applying this technique to anaerobic proteins provides insight int...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Berry, Luke, Patterson, Angela, Pence, Natasha, Peters, John W., Bothner, Brian
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5915355/
https://ncbi.nlm.nih.gov/pubmed/29713655
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2769
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