Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1(+) Tim-3(+) CD8(+) T Cells

Immune cells are important components of the tumor microenvironment and influence tumor growth and evolution at all stages of carcinogenesis. Notably, it is now well established that the immune infiltrate in human tumors can correlate with prognosis and response to therapy. The analysis of the immun...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Granier, Clémence, Vinatier, Emeline, Colin, Elia, Mandavit, Marion, Dariane, Charles, Verkarre, Virginie, Biard, Lucie, El Zein, Rami, Lesaffre, Corinne, Galy-Fauroux, Isabelle, Roussel, Hélène, De Guillebon, Eléonore, Blanc, Charlotte, Saldmann, Antonin, Badoual, Cécile, Gey, Alain, Tartour, Éric
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Cancer Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912389/
https://ncbi.nlm.nih.gov/pubmed/29553498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56606
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