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Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

We present a highly efficient way for the rapid preparation of a wide range of N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins. To achieve the desired structural diversity, the strategy began with the chemo-enzymatic synthesis of three...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Shivatare, Sachin S., Shivatare, Vidya S., Wu, Chung-Yi, Wong, Chi-Huey
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912354/
https://ncbi.nlm.nih.gov/pubmed/29443078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55855
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