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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

The creation of mutant lines by genome editing is accelerating genetic analysis in many organisms. CRISPR/Cas9 methods have been adapted for use in the African clawed frog, Xenopus, a longstanding model organism for biomedical research. Traditional breeding schemes for creating homozygous mutant lin...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Autor principal: Blitz, Ira L.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912334/
https://ncbi.nlm.nih.gov/pubmed/29443056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56035
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