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High-performance serial block-face SEM of non-conductive biological samples enabled by focal gas injection-based charge compensation

A longstanding limitation of imaging with serial block-face scanning electron microscopy is specimen surface charging. This charging is largely due to the difficulties in making biological specimens and the resins in which they are embedded sufficiently conductive. Local accumulation of charge on th...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Microsc
Prif Awduron: Deerinck, Thomas J., Shone, Tristan, Bushong, Eric A., Ramachandra, Ranjan, Peltier, Steven T., Ellisman, Mark H.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5910240/
https://ncbi.nlm.nih.gov/pubmed/29194648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jmi.12667
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