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High-performance serial block-face SEM of non-conductive biological samples enabled by focal gas injection-based charge compensation

A longstanding limitation of imaging with serial block-face scanning electron microscopy is specimen surface charging. This charging is largely due to the difficulties in making biological specimens and the resins in which they are embedded sufficiently conductive. Local accumulation of charge on th...

詳細記述

保存先:
書誌詳細
出版年:J Microsc
主要な著者: Deerinck, Thomas J., Shone, Tristan, Bushong, Eric A., Ramachandra, Ranjan, Peltier, Steven T., Ellisman, Mark H.
フォーマット: Artigo
言語:Inglês
出版事項: 2017
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5910240/
https://ncbi.nlm.nih.gov/pubmed/29194648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jmi.12667
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