Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

The pronuclear (PN) injection technique was first established in mice to introduce foreign genetic materials into the pronuclei of one-cell stage embryos. The introduced genetic material may integrate into the embryonic genome and generate transgenic animals with foreign genetic information followin...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Li, Rong, Miao, Jinxin, Fan, Zhiqiang, Song, SeokHwan, Kong, Il-keun, Wang, Yaohe, Wang, Zhongde
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Bioengineering
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908450/
https://ncbi.nlm.nih.gov/pubmed/29364218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56263
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