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Amplicon Competition Enables End‐Point Quantitation of Nucleic Acids Following Isothermal Amplification

It is inherently difficult to quantitate nucleic acid analytes with most isothermal amplification assays. We developed loop‐mediated isothermal amplification (LAMP) reactions in which competition between defined numbers of “false” and “true” amplicons leads to order of magnitude quantitation by a si...

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Detalhes bibliográficos
Publicado no:Chembiochem
Main Authors: Jiang, Yu Sherry, Stacy, Apollo, Whiteley, Marvin, Ellington, Andrew D., Bhadra, Sanchita
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5890436/
https://ncbi.nlm.nih.gov/pubmed/28628741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201700317
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