Amplicon Competition Enables End‐Point Quantitation of Nucleic Acids Following Isothermal Amplification

It is inherently difficult to quantitate nucleic acid analytes with most isothermal amplification assays. We developed loop‐mediated isothermal amplification (LAMP) reactions in which competition between defined numbers of “false” and “true” amplicons leads to order of magnitude quantitation by a si...

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Publicat a:Chembiochem
Autors principals: Jiang, Yu Sherry, Stacy, Apollo, Whiteley, Marvin, Ellington, Andrew D., Bhadra, Sanchita
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2017
Matèries:
Communications
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5890436/
https://ncbi.nlm.nih.gov/pubmed/28628741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201700317
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