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Amplicon Competition Enables End‐Point Quantitation of Nucleic Acids Following Isothermal Amplification

It is inherently difficult to quantitate nucleic acid analytes with most isothermal amplification assays. We developed loop‐mediated isothermal amplification (LAMP) reactions in which competition between defined numbers of “false” and “true” amplicons leads to order of magnitude quantitation by a si...

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Detalles Bibliográficos
Publicado en:Chembiochem
Main Authors: Jiang, Yu Sherry, Stacy, Apollo, Whiteley, Marvin, Ellington, Andrew D., Bhadra, Sanchita
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5890436/
https://ncbi.nlm.nih.gov/pubmed/28628741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201700317
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