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Real-time detection of isothermal amplification reactions with thermostable catalytic hairpin assembly

Catalytic hairpin assembly (CHA) is an enzyme-free amplification method that has previously proven useful in amplifying and transducing signals at the terminus of nucleic acid amplification reactions. Here, for the first time, we engineered CHA be thermostable from 37 °C to 60 °C and in consequence...

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書誌詳細
主要な著者: Jiang, Yu (Sherry), Li, Bingling, Milligan, John N., Bhadra, Sanchita, Ellington, Andrew D.
フォーマット: Artigo
言語:Inglês
出版事項: 2013
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3724415/
https://ncbi.nlm.nih.gov/pubmed/23647466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja4023978
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