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Real-time detection of isothermal amplification reactions with thermostable catalytic hairpin assembly
Catalytic hairpin assembly (CHA) is an enzyme-free amplification method that has previously proven useful in amplifying and transducing signals at the terminus of nucleic acid amplification reactions. Here, for the first time, we engineered CHA be thermostable from 37 °C to 60 °C and in consequence...
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| Glavni autori: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2013
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3724415/ https://ncbi.nlm.nih.gov/pubmed/23647466 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja4023978 |
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