Precise Cas9 targeting enables genomic mutation prevention

Here, we present a generalized method of guide RNA “tuning” that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism. We employ our methodology to generate an in vivo mutation prevention system in which Cas9 actively restricts the occurrence of undes...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Chavez, Alejandro, Pruitt, Benjamin W., Tuttle, Marcelle, Shapiro, Rebecca S., Cecchi, Ryan J., Winston, Jordan, Turczyk, Brian M., Tung, Michael, Collins, James J., Church, George M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2018
Schlagworte:
Biological Sciences
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5889643/
https://ncbi.nlm.nih.gov/pubmed/29555762
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1718148115
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