Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Komagataella phaffii (syn. Pichia pastoris) is one of the most commonly used host systems for recombinant protein expression. Achieving targeted genetic modifications had been hindered by low frequencies of homologous recombination (HR). Recently, a CRISPR/Cas9 genome editing system has been impleme...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Cell Biochem
Prif Awduron: Weninger, Astrid, Fischer, Jasmin E., Raschmanová, Hana, Kniely, Claudia, Vogl, Thomas, Glieder, Anton
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: John Wiley and Sons Inc. 2017
Pynciau:
Articles
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5887973/
https://ncbi.nlm.nih.gov/pubmed/29091307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcb.26474
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