RNA–protein interaction detection in living cells

RNA–protein interactions play numerous roles in cellular function and disease. Here we describe RNA–protein interaction detection (RaPID), which uses proximity-dependent protein labeling, based on the BirA* biotin ligase, to rapidly identify the proteins that bind RNA sequences of interest in living...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Ramanathan, Muthukumar, Majzoub, Karim, Rao, Deepti S, Neela, Poornima H, Zarnegar, Brian J, Mondal, Smarajit, Roth, Julien G, Gai, Hui, Kovalski, Joanna R, Siprashvili, Zurab, Palmer, Theo D, Carette, Jan E, Khavari, Paul A
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5886736/
https://ncbi.nlm.nih.gov/pubmed/29400715
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4601
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