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Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid
BACKGROUND: The MiniR1–1 plasmid is a derivative of the R1 plasmid, a low copy cloning vector. RESULTS: Nucleotide sequencing analysis shows that the MiniR1–1 plasmid is a 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication). The plasmid carries the repA, tap, copA an...
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| Publicado no: | BMC Microbiol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5883639/ https://ncbi.nlm.nih.gov/pubmed/29614952 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-018-1162-3 |
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