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DnaA dependent replication of plasmid R1 occurs in the presence of point mutations that disrupt the dnaA box of oriR.
We have found that DnaA dependent replication of R1 still occurred when 5 of the 9 bases in the dnaA box present in oriR were changed by site directed mutagenesis although the replication efficiency decreased to 20% and 70% of the wild-type origin in vitro and in vivo respectively. Additional mutati...
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| Auteurs principaux: | , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1992
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC312391/ https://ncbi.nlm.nih.gov/pubmed/1598213 |
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