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On the design of CRISPR-based single cell molecular screens
Several groups recently coupled CRISPR perturbations and single-cell RNA-seq (scRNA-seq) for pooled genetic screens. We demonstrate that vector designs of these studies are susceptible to ~50% swapping of guide RNA-barcode associations due to lentiviral template switching. We optimize a published al...
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| Publicado no: | Nat Methods |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5882576/ https://ncbi.nlm.nih.gov/pubmed/29457792 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4604 |
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