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Immobilization alters heparin cleaving properties of heparinase I

We report here a novel observation that immobilization of heparinase I on CNBr-activated Sepharose results in heparin degradation properties that are different from heparinase I in the free solution form. Studies over a range of pHs (5–8) and temperatures (5–50°C) as well as under batch and flow con...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Glycobiology
Hauptverfasser: Bhushan, Indu, Alabbas, Alhumaidi, Kuberan, Balagurunathan, Gupta, Ram B, Desai, Umesh R
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5881678/
https://ncbi.nlm.nih.gov/pubmed/28973365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/glycob/cwx074
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