Melting Proteins Confined in Nanodroplets with 10.6 μm Light Provides Clues about Early Steps of Denaturation

Ubiquitin confined within nanodroplets was irradiated with a variable-power CO(2) laser. Mass spectrometry analysis shows evidence for a protein “melting”-like transition within droplets prior to solvent evaporation and ion formation. Ion mobility spectrometry reveals that structures associated with...

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Publicado en:Chem Commun (Camb)
Main Authors: El-Baba, Tarick J., Fuller, Daniel R., Woodall, Daniel W., Raab, Shannon A., Conant, Christopher R., Dilger, Jonathan M., Toker, Yoni, Williams, Evan R., Russell, David H., Clemmer, David E.
Formato: Artigo
Idioma:Inglês
Publicado: 2018
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5871606/
https://ncbi.nlm.nih.gov/pubmed/29536995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7cc09829d
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