Development of light-activated CRISPR using photocleavable protectors of guide RNAs

The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. We developed a method to photocage the activity of the guide RNA called ‘CRISPR-plus’ (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivat...

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Bibliographic Details
Published in:Angew Chem Int Ed Engl
Main Authors: Jain, Piyush K., Ramanan, Vyas, Schepers, Arnout G., Dalvie, Nisha S., Panda, Apekshya, Fleming, Heather E., Bhatia, Sangeeta N.
Format: Artigo
Language:Inglês
Published: 2016
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5864249/
https://ncbi.nlm.nih.gov/pubmed/27554600
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201606123
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