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Development of light-activated CRISPR using photocleavable protectors of guide RNAs
The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. We developed a method to photocage the activity of the guide RNA called ‘CRISPR-plus’ (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivat...
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| Publicado no: | Angew Chem Int Ed Engl |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5864249/ https://ncbi.nlm.nih.gov/pubmed/27554600 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201606123 |
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