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Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy

As an image-based single-cell transcriptomics approach, multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows hundreds to thousands of RNA species to be identified, counted and localized in individual cells while preserving the native spatial context of RNAs. In MERFISH, RNAs...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Wang, Guiping, Moffitt, Jeffrey R., Zhuang, Xiaowei
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2018
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5859009/
https://ncbi.nlm.nih.gov/pubmed/29555914
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-22297-7
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