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Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq

Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While developed for individual Steinernema carpocapsae and Caeno...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Serra, Lorrayne, Chang, Dennis Z., Macchietto, Marissa, Williams, Katherine, Murad, Rabi, Lu, Dihong, Dillman, Adler R., Mortazavi, Ali
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5857950/
https://ncbi.nlm.nih.gov/pubmed/29564372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2729
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