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Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq
Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While developed for individual Steinernema carpocapsae and Caeno...
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| Publicado no: | Bio Protoc |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5857950/ https://ncbi.nlm.nih.gov/pubmed/29564372 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2729 |
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