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Effective microtissue RNA extraction coupled with Smart-seq2 for reproducible and robust spatial transcriptome analysis

Spatial transcriptomics is useful for understanding the molecular organization of a tissue and providing insights into cellular function in a morphological context. In order to obtain reproducible results in spatial transcriptomics, we have to maintain tissue morphology and RNA molecule stability du...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Yamazaki, Miki, Hosokawa, Masahito, Arikawa, Koji, Takahashi, Kiyofumi, Sakanashi, Chikako, Yoda, Takuya, Matsunaga, Hiroko, Takeyama, Haruko
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7184581/
https://ncbi.nlm.nih.gov/pubmed/32341421
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-63495-6
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