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Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases

The unique translocation and DNA unwinding properties of DNA helicases can be concealed by the stochastic behavior of enzyme molecules within the necessarily large populations used in ensemble experiments. With recent technological advances, the direct visualization of helicases acting on individual...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Pavankumar, T.L., Exell, J.C., Kowalczykowski, S.C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5854184/
https://ncbi.nlm.nih.gov/pubmed/27793277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2016.09.010
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