Complementarity of assembly-first and mapping-first approaches for alternative splicing annotation and differential analysis from RNAseq data

Genome-wide analyses estimate that more than 90% of multi exonic human genes produce at least two transcripts through alternative splicing (AS). Various bioinformatics methods are available to analyze AS from RNAseq data. Most methods start by mapping the reads to an annotated reference genome, but...

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Udgivet i:Sci Rep
Main Authors: Benoit-Pilven, Clara, Marchet, Camille, Chautard, Emilie, Lima, Leandro, Lambert, Marie-Pierre, Sacomoto, Gustavo, Rey, Amandine, Cologne, Audric, Terrone, Sophie, Dulaurier, Louis, Claude, Jean-Baptiste, Bourgeois, Cyril F., Auboeuf, Didier, Lacroix, Vincent
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5844962/
https://ncbi.nlm.nih.gov/pubmed/29523794
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-21770-7
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