CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae

Cpf1 represents a novel single RNA‐guided CRISPR/Cas endonuclease system suitable for genome editing with distinct features compared with Cas9. We demonstrate the functionality of three Cpf1 orthologues – Acidaminococcus spp. BV3L6 (AsCpf1), Lachnospiraceae bacterium ND2006 (LbCpf1) and Francisella...

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Udgivet i:Yeast
Main Authors: Verwaal, René, Buiting‐Wiessenhaan, Nathalie, Dalhuijsen, Sacha, Roubos, Johannes A.
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2017
Fag:
Research Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5836994/
https://ncbi.nlm.nih.gov/pubmed/28886218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.3278
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