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Identification of biosynthetic gene clusters from metagenomic libraries using PPTase complementation in a Streptomyces host

The majority of environmental bacteria are not readily cultured in the lab, leaving the natural products they make inaccessible using culture-dependent discovery methods. Cloning and heterologous expression of DNA extracted from environmental samples (environmental DNA, eDNA) provides a means of cir...

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Bibliographic Details
Published in:FEMS Microbiol Lett
Main Authors: Bitok, J. Kipchirchir, Lemetre, Christophe, Ternei, Melinda A., Brady, Sean F.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2017
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5827622/
https://ncbi.nlm.nih.gov/pubmed/28817927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsle/fnx155
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