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Mapping gene clusters within arrayed metagenomic libraries to expand the structural diversity of biomedically relevant natural products
Complex microbial ecosystems contain large reservoirs of unexplored biosynthetic diversity. Here we provide an experimental framework and data analysis tool to facilitate the targeted discovery of natural-product biosynthetic gene clusters from the environment. Multiplex sequencing of barcoded PCR a...
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| Main Authors: | , , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3718090/ https://ncbi.nlm.nih.gov/pubmed/23824289 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1222159110 |
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