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Reconstituted System for the Examination of DNA Repair Synthesis in Homologous Recombination

In homologous recombination (HR), DNA polymerase δ-mediated DNA synthesis occurs within the displacement loop (D-loop) that is made by the recombinase Rad51 in conjunction with accessory factors. We describe in this article the reconstitution of the D-loop and repair DNA synthesis reactions using pu...

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Publicado en:Methods Enzymol
Main Authors: Kwon, Youngho, Daley, James M., Sung, Patrick
Formato: Artigo
Idioma:Inglês
Publicado: 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5823693/
https://ncbi.nlm.nih.gov/pubmed/28645374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.03.021
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