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Nuclear magnetic resonance‐based determination of dioxygen binding sites in protein cavities

The location and ligand accessibility of internal cavities in cysteine‐free wild‐type T4 lysozyme was investigated using O(2) gas‐pressure NMR spectroscopy and molecular dynamics (MD) simulation. Upon increasing the concentration of dissolved O(2) in solvent to 8.9 mM, O(2)‐induced paramagnetic rela...

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Vydáno v:Protein Sci
Hlavní autoři: Kitahara, Ryo, Sakuraba, Shun, Kameda, Tomoshi, Okuda, Sanshiro, Xue, Mengjun, Mulder, Frans A.A.
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5818741/
https://ncbi.nlm.nih.gov/pubmed/29271012
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3371
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