Analysis of O(2)-binding Sites in Proteins Using Gas-Pressure NMR Spectroscopy: Outer Surface Protein A

Internal cavities in proteins produce conformational fluctuations and enable the binding of small ligands. Here, we report a NMR analysis of O(2)-binding sites by O(2)-induced paramagnetic relaxation enhancements (PREs) on amide groups of proteins in solution. Outer surface protein A contains a nong...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Kawamura, Takahiro, Wakamoto, Takuro, Kitazawa, Soichiro, Sakuraba, Shun, Kameda, Tomoshi, Kitahara, Ryo
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2017
Assuntos:
Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5425398/
https://ncbi.nlm.nih.gov/pubmed/28494953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2017.03.029
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