Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae

Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be q...

Full description

Saved in:
Bibliographic Details
Published in:Bio Protoc
Main Authors: Shashkova, Sviatlana, Wollman, Adam JM, Hohmann, Stefan, Leake, Mark C
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2018
Subjects:
Methods Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5802385/
https://ncbi.nlm.nih.gov/pubmed/29430484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2710
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items