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Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae

Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be q...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Bio Protoc
Päätekijät: Shashkova, Sviatlana, Wollman, Adam JM, Hohmann, Stefan, Leake, Mark C
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Bio-Protocol 2018
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5802385/
https://ncbi.nlm.nih.gov/pubmed/29430484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2710
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