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Efficient strategy for introducing large and multiple changes in plasmid DNA
While the QuikChange site-directed mutagenesis method and its later modifications are extremely useful and simple, they suffer from several drawbacks. Here, we propose a new method, named LFEAP mutagenesis (Ligation of Fragment Ends After PCR) for creating various mutations in plasmid by leveraging...
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| Publicado no: | Sci Rep |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5789069/ https://ncbi.nlm.nih.gov/pubmed/29379085 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-20169-8 |
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