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AFEAP cloning: a precise and efficient method for large DNA sequence assembly

BACKGROUND: Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), which allow...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Zeng, Fanli, Zang, Jinping, Zhang, Suhua, Hao, Zhimin, Dong, Jingao, Lin, Yibin
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5686892/
https://ncbi.nlm.nih.gov/pubmed/29137618
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-017-0394-x
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