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Direct detection of lysine side chain NH(3)(+) in protein-heparin complexes using NMR spectroscopy
Two NMR observables, N(ζ)H(3)(+) peak in the HISQC spectrum and N(ζ) chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to eithe...
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| Publicado no: | Analyst |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5788726/ https://ncbi.nlm.nih.gov/pubmed/29292440 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7an01406f |
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