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Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts

Alcohol oxidase I (AOX1) promoter is the most popular but strictly-regulated methanol inducible promoter for heterologous protein expression in Pichia pastoris. In recent years, AOX1 promoter libraries have been developed with deletion or insertion methods. The present research manipulated poly (dA:...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Yang, Jun, Cai, Haiming, Liu, Jie, Zeng, Min, Chen, Jiawei, Cheng, Qingmei, Zhang, Linghua
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5780452/
https://ncbi.nlm.nih.gov/pubmed/29362428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-19831-y
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