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Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts

Alcohol oxidase I (AOX1) promoter is the most popular but strictly-regulated methanol inducible promoter for heterologous protein expression in Pichia pastoris. In recent years, AOX1 promoter libraries have been developed with deletion or insertion methods. The present research manipulated poly (dA:...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Yang, Jun, Cai, Haiming, Liu, Jie, Zeng, Min, Chen, Jiawei, Cheng, Qingmei, Zhang, Linghua
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5780452/
https://ncbi.nlm.nih.gov/pubmed/29362428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-19831-y
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